Definitely. While I do not use the exact file / folder structure from the library on my PC, I do have a structure on the thumb drives in our vehicles to make it easier to know what's on there. My comment was more for informing that the file structure has nothing to do with the vehicle's music...
My parents used to own a Ford Escape (cannot remember the year) that had no issues playing MP3 files from a thumb drive.
Ron, if you have a USB memory stick laying around, why not just copy some music onto it and give it a try?
As for file structure, you shouldn't need to maintain the same...