Short story: No, but that doesn't mean it's not coming out.
Long story: Originally the set was supposed to come out August or September, alongside the other Warner TV Blu-rays (T:SCC, Fringe, Supernatural, etc.), but the ongoing confusion about the show's status--eventually, and thankfully, renewed at the last minute--shelved the plans. Currently, the 3rd season is tentatively set to begin March 2010 with the distinct possibility of an earlier start if NBC's lineup nosedives. I believe the showrunners were asked after the Comic-Con panel when the DVDs (and presumably the Blu-rays) would hit, and they said that they're linked to the 3rd season premiere.
I hate it when they do that. They never give you enough time to rewatch the previous season between when the DVDs hit and when it airs. Dollhouse is the only exception (from what I watch) that I can think of.
Warner Home Video has announced that it will release 'Chuck: The Complete Second Season' on Blu-ray on January 5, 2010, day-and-date with the DVD. The 22-episode season will come in four discs. As usual with television content from this studio, only a Dolby Digital track will be included, with no lossless audio option.
Special features include:
Truth, Spies and Regular Guys: Exploring the Mythology of Chuck
Dude in Distress: Explore Some of This Season's Best Action Sequences
Chuck Versus the Webisodes: Web-Originated Minifeaturettes
Chuck: A Real-Life Captain Awesome's Tips for Being Awesome
John Casey Presents: So You Want to Be a Deadly Spy?